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Yet, these experiments have involved use of native TEs without attempting to promote transposition efficiency or controlling the transposition specifically in germinal cells.
To promote transposition efficiency and control transposition specifically in germinal cells, we assessed the use of an inducible transposon, COKC, to create DH mutant rice plants.
A second pathway uses TnsABC + TnsE to promote transposition to sites unrelated to attTn7.
IS1 transposases with an amino acid substitution in the HTH or ZF motif lose the ability to promote transposition, indicating that 2 domains are responsible for TIR-specific DNA binding in promoting transposition.
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SB was first shown to promote germline transposition and transgenesis in mice [47], [48] and was further developed as a germline mutagenesis tool [47], [49].
In contrast, transcription of an 8-triplet AATATT repeat in either orientation on plasmids did not produce significant changes in cell morphology and did not promote IS1E transposition events.
The existence of large families of highly similar intron sequences in these genomes suggests that certain intron sequences are much more likely to be transposed than others and that specific sequence patterns might promote intron transposition [ 273].
H-NS was found to be a key protein for these processes; presumably inactivating triplet repeats transcription by differently binding to the repeat arrays and then promoting IS1E transposition (Figure 6).
Evidently then, the initial contact of SecA with SecYEG promotes the transposition of the PPXD, most probably due to its interaction with cytoplasmic loop C5 of SecY, between TMSs 8 and 9 [ 7].
TEs usually encode the genes that promote their own transposition, but many non-autonomous elements use the transposition machinery of close relatives or unrelated elements instead.
Gyrase binding sites have been identified in Mu and some other Mu-like phages, and are thought to promote the replicative transposition process that occurs during Mu lytic growth.
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