1,4-butanediol was autoclaved separately and added aseptically to the nitrogen-free MM (1% v/v) as the sole carbon source to promote synthesis of the copolymer.
While the latter AChEs exclusively promote synthesis of syn-substituted triazoles, the best Drosophila AChE triazole inhibitors were always anti-substituted.
After 24 h, the cells were harvested by centrifugation at 4000 g, 16°C for 10 min. To promote synthesis of PHA, the harvested cells were transferred into the second step medium, which contained 100 mL of nitrogen-free mineral medium (MM) (pH 7.0).
Although it has been demonstrated that a low oxygen concentration could promote synthesis of the extracellular matrix by IVD cells in the in vitro culture, isolation, expansion, and cultivation of IVD cells under classical tissue culture O2 saturation could still be detrimental.
In tissue culture TDZ could promote synthesis or accumulation of endogenous cytokinins [ 27].
IGF-1 is known to promote synthesis of collagen type II, a major component of the cartilage matrix.
Endothelins promote synthesis of ECM proteins by epithelial cells and fibroblasts, myofibroblast differentiation, and proliferation of mesenchymal cells 15, 16.
TGFβ has been previously reported to promote synthesis of vascular endothelial growth factor α (VEGFα) (Nakagawa et al., 2004).
Hypoxia inducible factors HIF-1α and HIF-2α are known to promote synthesis of cartilaginous ECM in chondrocytes through induction of the Sox9 pathway [ 43, 44].
Autologous preparations that are rich in GFs induce cell proliferation and promote synthesis of angiogenic factors during the healing process [ 20].
Subsequently, the prostaglandins PGE2 and PGD2 are generated in a cyclooxygenase-dependent way to promote synthesis of lipid mediators with anti-inflammatory activity, such as the lipoxins.
