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Further in vitro experiments using functionalized microspheres with surface integrated RGD peptide activate co-cultured skeletal populations in pellets and promote secretion of extracellular matrix collagens and human osteocalcin.
These functionally compromised mitochondria failed to promote secretion of DAF-28/insulin.
It has been postulated that Rac1 signals downstream of α6β4 integrin and p38MAPK in thymic epithelial cells to promote secretion of IL6 upon thymocyte contact [25].
It has also been suggested that pilin glycosylation may promote secretion of the soluble (S) pilin subunits [ 71].
In addition, increased palmitic acid in VLDL-triacylglycerols has been shown to promote secretion of proinflammatory mediators by human smooth muscle cells [ 76].
Nie et al., have also demonstrated the capacity of GIP to activate inflammatory response and promote secretion of pro-inflammatory cytokines and chemokines in cell culture adipocytes [ 35].
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Senescent cells secrete HMGB1 promoting secretion of inflammatory cytokines (TNFα, IL-1β and IL-6) by macrophages [ 55].
These studies indicate that while the SP is very important in promoting secretion of the scFv, it had little influence on increasing scFv secretion levels even when both the TEV and the KDEL sequences significantly increased overall protein levels.
More importantly, AMF inoculation ameliorated inhibitory effects of ZnONPs by promoting secretion of glycoprotein called glomalin a potent metal chelator within the rhizosphere, which significantly reduced (by almost half) Zn uptake by root and subsequent translocation to the shoot.
Exosomes are small secreted membrane-bound particles measuring 30 to 120 nm in diameter, that have been shown to play important roles in cell-cell signaling and cellular communication, promoting secretion of growth factors, cytokines, and angiogenic factors by stromal cells, proliferation of endothelial cells, and metastasis (reviewed in ref. 5).
Moreover, using cocultured cells (DPSCs/CCN3 and DPSCs) in vitro and the cocultured cells-poly (lactic-co-glycolic acid) (PLGA) scaffold complex in vivo, we demonstrated that CCN3 was capable of promoting mineralization in a non-cell autonomous manner through promoting secretion of BMP2.
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