Samples were pretreated to promote antigen retrieval with Target Retrieval Solution (DAKO, Carpinteria, CA, USA).
For CD31 retrieval, sections were incubated in trypsin to promote antigen retrieval and blocked with normal goat serum for 30 min.
For NPY and MCH antisera, sections were placed into small jars of citrate buffer (10 mM, pH6.0) and placed in a 650 W microwave on high power for ∼12 seconds to promote antigen retrieval prior to staining.
Samples were then quenched in 3% hydrogen peroxide (Macron Fine Chemicals, 5240-02) and pretreated with the steam of DIVA solution (Biocare Medical, DV2004MX) to promote antigen retrieval.
Samples were then quenched in 1% hydrogen peroxide and pretreated with steam from citrate buffer (Vector Laboratories, Burlingame, CA, USA; H-3300) to promote antigen retrieval.
Proteins remain because they express signals that prevent them from leaving the ER or promote their retrieval from the Golgi apparatus.
Increased FC between HIP-MGB and HIP-IC could facilitate the formation or stabilization of a memory trace for tinnitus, assign a spatial location to the phantom sound within or outside the head, or promote the retrieval of previously stored auditory memories (De Ridder et al., 2006; Ulanovsky and Moss, 2008).
Sections were pretreated to promote antigen retrieval by steam in DIVA/citrate buffer (pH 7.0) solution, and quenched in 0.3% H2O2; then slides were incubated in 10% normal goat serum, followed by an anti-occludin antibody overnight at 4°C [1 80 dilution in phosphate-buffered saline (PBS)].
Participants performed a standard version of a 2-back working memory task with strong attentional demands, and a cued version that promoted memory retrieval.
In this context, the EU directive Infrastructure for Spatial Information in Europe [INSPIREa] is an ambitious initiative to promote standardised data retrieval.
