We have considered that the zinc influx may promote loading of zinc finger proteins.
It notably contains the di-acidic sequence Asp-Ile-Asp previously reported to promote loading of the permease into COPII vesicles [23].
It is not clear how this form of NMD is promoted, but one idea is that unknown 3′-UTR-associated factors prevent PABP1 from interacting with eRF3 and promote loading of UPF1 to initiate NMD [ 33].
Consistent with this notion, we found that several parB mutants, which are defective in the ability to form large nucleoprotein complexes and to spread from parS sites, fail to promote loading of Smc ScpAB onto the chromosome.
Although we could not identify any Giw1p homolog in non- Tetrahymena species, a functionally similar "lid" could also exist in other eukaryotes, and Hsp90 chaperone activity might be used to displace the lid to promote loading.
This idea seems to contrast the previously proposed mechanism of the loading of miRNAs and siRNAs into Argonaute proteins in mammals, flies, and plants, in which ATP-bound Hsp90 is suggested to promote loading by inducing a conformational change of the Argonaute proteins to accommodate a bulky small RNA duplex (Kawamata & Tomari, 2010).
Dynamic anterior plates have been popularized to promote cervical spine fusion by allowing controlled settling, thereby promoting load sharing across the construct.
The fragment containing amino acids 1 254 did not support loading (Fig 5A, N1), and the fragment spanning the amino acids 196 381 only weakly promoted loading in the COI12 KO cell lysate (Fig 5A, N3).
In addition to the ATP-independent loading-enhancing activity, an Hsp90- and ATP-dependent activity promotes loading of scnRNAs into Twi1p in Tetrahymena, as in many other eukaryotes (Fig 6A C).
Using energy saving replacement or reconstruction for electrical equipment and new energy-saving technology, EPP could reduce the electricity load and promote peak load shifting [44].
