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These results proved that ARTP mutagenesis is a promising mutation breeding tool for industrial applications.
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Favorable (i.e., negative) stability and affinity ΔΔ G values calculated for all four interfaces indicated promising mutations.
Sequencing the whole genome of one sample yielded 10 promising mutations, eight of which were "private" mutations, meaning that they had not been detected in previous samples.
Promising mutations can then be evaluated in detail in the system.
By combining the criteria of energy stabilization and sequence variability, we selected nine most promising mutations (Table 2).
The biochemical characterization of the enzyme variants revealed great differences in catalytic activity and stability and allowed the combination of the most promising mutations to potentially give an unglycosylated, active HRP C1A variant useful for medical applications.
In Section S1.7 in the Supporting Information we describe an approach to reduce the number of candidate mutations using functional mode analysis;[ 16, 17] this approach successfully identified the positions of the most promising mutations based on ten 100 ns molecular dynamics (MD) simulations of unbound ubiquitin.
For the 15 most promising mutations, the destabilization is less than 23.6 kJ mol−1, the folding free energy of the wild-type ubiquitin.[ 19] An additional computational validation was performed using umbrella sampling simulations (for computational details see the Supporting Information).
If this thing isn't growing its own hair, it's at the very least not promising anything about mutation, retching, chaos.
Clinical trials have shown drugs in this class to be promising for BRCA mutation carriers.
Early mutations are promising therapeutic targets, and late mutations are important in metastasis.
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