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Using phosphate-affinity gel electrophoresis [37], Borealin displayed a prominent mobility shift in mitotic samples of nocodazole-treated cells (Fig. 6D).
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Strikingly, a prominent different mobility profile was observed for mFBs during co-culture.
Both the defined oligomers and the generalized intra-lane background (the latter likely reflecting BiP interactions with unfolded client proteins) were diminished and replaced by a prominent high mobility species (labeled 'B' form, Figure 8A, lanes 1 and 2).
The other distinct feature of the G173R mutation is that it causes a prominent electrophoretic mobility shift in RPGRORF15, but not RPGR1 19, and its decreased stability that is reflected by the much lower expression level of the mutant versus the wild-type protein.
In addition, four prominent bands with an mobility or apparent mass of 43-, 37-, 29- and 24-kDa were present in elute fractions of G1/pCRASP-3 and G1/pCRASP-5.
From this perspective, autonomy and mobility become prominent, as well as the impact of the technology on users' social identity.
Under both conditions the phospho-sensitive antibody detected a prominent band with an apparent mobility of approximately 26 kDa in extracts from cells expressing AQP2-WT corresponding to the expected mobility of unglycosylated AQP2 (Fig. 5b, lane 1 and 2).
Extensive traditional livestock production associated with herd mobility is still prominent in the nomadic land use site where land has not been sub-divided.
Reduced drop mobility is more prominent for drop sizes that are comparable to the tube diameter and is maximum when mass transfer to and from the interface is inhibited.
It also emerges that, while for the whole sample in less than 8 years mobility becomes the prominent form of adjustment, for the pre-crisis period, it takes more than 11 years for mobility to overtake activity rates as the most relevant adjustment channel.
Analysis by SDS-PAGE and Coomassie-blue staining of extracts from cells infected with these baculoviruses revealed the presence of two prominent bands with the electrophoretic mobility expected for both GFP (Figure 3A, lane 5) and GFP-IC (lane 6, GFP-IC), which were not present in uninfected or wild-type baculovirus-infected cells (lanes 1 and 2).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com