Exact(9)
The most prominent amplification method used was developed by the Eberwine lab in 1990: cDNA synthesis is started with an oligo dT) primer containing a T7 RNA polymerase promoter.
The primer pair that produced a single and prominent amplification product was taken for further analysis.
The band patterns were different in all 27 PCR reactions, suggesting that there was no single highly prominent amplification product.
However, the prominent amplification of the region may indicate a potential role for this variant in lymphomagenesis.
The most prominent amplification of Shh response was seen when Sema3F was added to cells in the presence of a high concentration of Shh (2 μg/ml).
However, along with a 690 bp PCR fragment corresponding to mRNA lacking exon 4, an additional prominent amplification product, a 562 bp fragment, was detected in Hunk−/− animals.
Similar(51)
A large amplification, and a phase delay of the Rayleigh wave, is prominent in the water model.
RT-PCR assays contained quite prominent aspecific amplification signals, which are likely due to the high number of PCR cycles needed to detect sufficient signal.
In summary, this set of breast cancer cell lines displaying prominent DNA amplifications should facilitate discovery and functional analysis of genes and signal transduction pathways contributing to breast cancer development.
A prominent example is amplification of the N-myc proto-oncogene, the copy number of N-myc is substantially elevated in some human tumors and far exceeds the normal two-copy state [1], [2].
Monoclonal gene rearrangements were identified as prominent, single-sized amplification products; the base pair length was recorded for each fraction.
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