Sentence examples for prolonged extension from inspiring English sources

In other cases, prolonged extension has resulted in an increased crustal geotherm, and relatively high-temperature metamorphism and magmatism is thus directly related to the extensional event.

Concerning the distribution pattern of the neurite lengths, the population of long-neurite-bearing cells was much smaller than the population under optimal conditions, whereas the population of short-neurite-bearing cells decreased upon a transient (1 h) second stimulation, indicating that sustained stimulation was required for the prolonged extension of the neurites (see Figure S2D,G,I).

The final cycle had a prolonged extension at 72 °C for 5 mins.

The final step was a prolonged extension of 5 min at 72°C.

A prolonged extension step was carried out at 72°C for 7 minutes.

We overcame this problem with a final and prolonged extension step, so that all of the partial products are converted to full-length products.

prolonged overlap extension PCR.

The assumption that several hundred thousand data points (and, by extension, prolonged scanning times) are required to produce legitimate data is not based on real world testing.

The standard GP5+/GP6+ amplification cycles comprise 1 min at 94°C, 2 min at 40°C, and 1.5 min at 72°C, with the final extension step prolonged to 4 min to ensure complete amplification of the 140 150 base pair products [ 6, 7].

Consistent with recent research using data for other countries (Card et al. 2007), the main estimated impact of the recent U.S. benefit extensions was prolonged labor force attachment rather than reduced job finding.

The PCR reaction included one cycle of initial amplification (at 94°C for 5 min, 56°C for 3 min, 72°C for 2 min), followed by 22 to 32 cycles at 94°C for 1 min, annealing at 56°C for 1 min, and extension at 72°C for 0.5 min. The final extension reaction was prolonged to 10 min at 72°C.