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In the late stage, the I t curve values become to increase, the peak areas extend, and the degradation times prolong with the increasing of TBA concentration.
The delaying and the total time of the forming process prolong with the lowering of the frequency, and the optimum frequency is found that corresponds to the largest deformation and the greatest plastic strain energy of the tube.
Coverslips were then mounted using Prolong with DAPI.
Fluorescent slides were cover-slipped using Prolong with DAPI (Invitrogen, Carlsbad, CA).
Fluorescent slides were cover-slipped using Prolong with DAPI (Invitrogen).
After three final washes in PBS, the cells were mounted with Prolong with DAPI (4,6-diamidino-2-phenylindole; Invitrogen) to counterstain for the nuclei.
Similar(53)
The coverslips were washed three times and mounted with Prolong gold with DAPI (Invitrogen, Carlsbad, CA).
Nuclei were counterstained and mounted with Prolong Gold with DAPI (Invitrogen).
Sections were mounted with Prolong Gold with DAPI (Invitrogen).
Invading cells were then stained and mounted on slides with Prolong Antifade with DAPI (Molecular Probes).
OVs were mounted with Prolong Gold with DAPI (Invitrogen) and visualized on a confocal microscope (Leica, TCS SP2).
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