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In a similar fashion, treatment with U0126 also blocked the proliferative (data not shown) and prosurvival effects of IGF-1 and restored the cytotoxic action of 4-OHT and MIF, which included enhancing ROS levels and increasing the percentage of mitochondrial membrane depolarization (see Additional files 2a and 2b).
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Although these cell lines express wild-type Notch1 on their cell surface (Figure 5), treatment with anti-Notch1 antibodies at saturating concentrations (0.1 µM) did not affect their proliferative capacity (data not shown).
Of the 16 subjects only 5 were immunized (p = 0.045), 4 tolerized (p = 0.036) and 8 showed no change in the CD4+ T cell proliferative response (data not presented), when stimulation with the partners' monocytes were compared with those of 3rd party cells (Figure 3C, Figure S2A).
IL-7 alone did not elicit any proliferative response (data not shown).
Afterwards, 3D-cultured HMECs predominantly generated spheres, suggesting a loss of regenerative, but not proliferative, capacity (data not shown).
Levels of anti-BAFF, as measured by ELISA, did not show an association with proliferative nephritis (data not shown).
CMs cultured on either glass or on the stiff (2 MPa) substrate presented similar proliferative capacities (data not shown).
These constructs were targeted normally to the Golgi apparatus, and the cell lines showed no morphological or proliferative defects (data not shown).
No significant correlation was found between the ratio of CD4+ to CD8+ cells and tumour stage or proliferative response (data not shown).
E2, E3, BPA, or HPTE did not increase Ki67 in KIKO epithelial cells, indicating a lack of proliferative stimulation (data not shown) and emphasizing a requirement for the DNA-binding function of ERα for uterine growth.
Combinatorial treatment with 1 nM concentrations of estrogen plus ERβ-specific agonists did not result in greater anti-proliferative effects (data not shown).
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