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Adenovirus treatment was performed at three different stages: (1) proliferating myocytes at ∼50% confluency (proliferation stage); (2) proliferating myocytes at ∼90% confluency before induction of differentiation (fusion stage); and (3) differentiating cells 3 days after the induction of differentiation (differentiated stage).
"Right now we're in the proliferation stage," she explains.
This may explain why the isovitexin first increase during proliferation stage, and then decrease.
On the contrary, the highest isovitexin yield was detected in proliferation stage.
Alkaline phosphatase activity is also significantly elevated on porous scaffolds following the proliferation stage.
Next, BFP-1 released from pep@MSNs induced hMSCs osteo-differentiation after the proliferation stage.
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This includes body structures and the cell reproduction (aka, multiple proliferation stages) of all creatures involved.
Moreover, we confirmed that LV efficiently transduced primary myoblasts both at the proliferation (myoblast) stage and at myotubes stage [ 34, 35].
Concomitantly with the increase of the adult epithelial cells in number due to active proliferation at stage 62, the number of LGR5-positive cells increased (Fig. 5D, D').
Ki67 staining demonstrated similar patterns of proliferation by stage and genotype (supplementary material Fig. S1O).
Cell proliferation and stage of the cell cycle were quantified by trypan blue counts and flow cytometry, respectively.
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