3D scaffolds obtained with 10 and 12.5% polymer solutions (3D-2 and 3D-3, respectively) were identified as the most suitable construct for the cell attachment and proliferation presenting pore size ranged between 100 and 400 μm, high porosity (77 78%) and well interconnected pores.
a Proliferation presented as the number of cells per square millimeter.
Western blotting analysis of KI-67 (Abcam, France), a reliable endogenous marker of cell proliferation present throughout the entire cell cycle phase except G0 [27], was carried out using discontinuous (3 8%) SDS-PAGE precast gels (Invitrogen).
Epidermal cell proliferation (presented as BrdU labeled cells/mm of basement membrane) was determined as follows: Basement membrane (BM) length was determined in skin sections and the corresponding number of BrdU labeled cells of the respective interfollicular epidermal sector was counted.
The same argument holds true for the mesenchymal proliferation present in the patient's GI tract.
Ki67 is a marker of proliferation present throughout all active phases of the cell cycle 25.
For values of proliferation presented in Figure 3E, paired T tests were used.
Next, we looked for molecular markers of increased epithelial proliferation present in the IKMV glands.
(D ) Adipogenitc progenitor cell proliferation presented as percent of BrdU + cells by FACS analysis under normal culture condition (n = 3).
