In addition to the original screen, we employed multiple experimental approaches, such as luciferase validation studies, FACS analysis, microarray and Cyquant cell proliferation methods to examine the functional relevance of selected NATs.
This was accomplished by adding the saline extracts to HGFs and using a widely accepted viability and proliferation test method, methylthiol tetrazolium (MTT) assay (Wikipedia, 2012), to obtain the results.
One possible explanation is that the label-free xCELLigence assay provided a more sensitive cell proliferation detection method than the traditional end point assays, and the end point cell proliferation assays at fixed time points may have missed the subtle changes in cell proliferation.
Multivariate survival analyses were done with the Cox proportional hazards model, entering the following covariates: automated assessment of Ki-67 extent of staining or visually assessed Ki-67 proliferation index, method of tumour detection, tumour size in centimetres, number of metastatic lymph nodes, histological grade, and age at diagnosis.
Despite a proliferation of methods for the synthesis of qualitative research, less attention has focused on how to integrate these syntheses within intervention effectiveness reviews.
As we argued in the preceding sections, the history of twentieth-century genetics is characterized by a proliferation of methods for the individuation of genetic components, and, accordingly, by a proliferation of gene definitions.
This has led to the proliferation of methods for imputing four-digit genotype from limited sequencing data by utilizing known allele frequencies in various populations [ 19- 21].
In addition to morphological characteristics and measuring endothelial proliferation, imaging methods such as DCE, MRI, or PET (positron emission tomography) probes for hypoxia will be important to monitor vessel co-option/angiogenesis status in tumors prior to and during treatment.
In this normal condition, it can be difficult to measure proliferation with methods that utilize the detection of proteins associated with cell cycle activation (such as PCNA, Ki67) at a single time point (i.e., the time of animal sacrifice or tissue sampling).
