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The tritiated thymidine incorporation into the cells, which reflects the proliferation level, was measured by a liquid scintillation counter.
The proliferation level through the truncated mutant was even higher than that of non-transduced HSCs with the stimulation of a native cytokine, thrombopoietin.
Proliferation level in cells was evaluated using the Cell Titer96 non-radioactive cell proliferation assay kit (Promega) according to manufacture's protocol.
Before testing the wound closure, we examined the proliferation rate of the periostin-HaCaT transfectant and found no significant difference in the proliferation level between it and the cells transfected with the control vector (Figure S1).
However, this is the first time that the opposite effects of RA and EGF+bFGF are shown at the proliferation level in parallel to the Erk activation in a serum free context.
In contrast, when the cells had lost their ability to progress through S-phase after 3 5 days in 2D, then transferred to 3D BM-matrix for 2 days before being replated back onto 2D collagen, the proliferation level was much lower with less than 10% of the cells being EdU positive (Fig. 5b and c).
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No pronounced influence on cell proliferation levels could be observed after treatment with this antibody.
Titanium oxides containing enriched F− ions stimulate cell attachment, whereas high proliferation levels of cells are observed in phosphate incorporated titanium oxides.
Metabolic adaptations are essential during tumour growth to maintain the high proliferation levels exhibited by cancer cells.
It is important to note that the low adhesion and proliferation levels of osteoblasts on BC were not simply due to a cytotoxic effect.
The low adhesion and proliferation levels of osteoblasts were significantly (p<0.05) improved by the addition of HA (Blomquist et al.,1979).
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