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This intermediate step was used for cell proliferation in order to start the aerobic cultivations with high levels of biomass.
Over the evolutionary timescale, organisms with the ability to renew somatic tissue needed to evolve tumor suppressor mechanisms to regulate and control cellular proliferation in order to avoid unregulated cell growth leading to cancer.
We have examined the effects of 1,25(on)2D3 on the differentiation of porcine MSC under culture conditions designed to promote proliferation in order to attempt to mimic the conditions in young, rapidly growing animals.
The main objective of DES was to reduce a vascular inflammatory response and cell proliferation in order to eliminate an excessive hyperplastic healing response observed in BMSs to diminish a chance of ISR occurance (Abizaid and Costa Jr., 2010; Simard et al. 2014; Verheye et al. 2014).
Bone marrow was cultured in the same flask with the same media, to allow adherent cell proliferation in order to obtain constitutional fibroblasts from the patient.
A range of other genes were investigated both in vivo and in vitro associated with RPE proliferation in order to identify gene expression differences between the centre and the periphery.
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Ki67 and proliferating cell nuclear antigen (PCNA) were used as cell proliferation markers to further evaluate the proliferation rate in order to confirm the result of BrdU incorporation.
We therefore performed a BrdU proliferation assay in order to determine whether a subtle proliferation defect was present when RUNX1 level was lower in FPD-AML patients.
We performed FACS analysis and SRB cell proliferation assay in order to determine the role of EIF5A2 in apoptosis and cell proliferation (Supplementary Figure S8).
Cells were used at passage 4 or 5 for cell migration or proliferation assays in order to minimize the influence of genetic alterations and senescent changes in the cellular morphology.
4) The authors should be more consistent in the use of cell proliferation assays in order to facilitate comparisons between experiments within the manuscript.
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