Proliferation was measured in counts per minute (cpm) by a standard 3H thymidine incorporation assay, and maximum proliferative response was calculated on an index between the unstimulated control and the maximum proliferation counts.
These values are in agreement with the increase in proliferation markers, counting of cytokinesis events, and clonal expansion of CMs cultured on the soft matrices.
Interestingly, addition of CITCO resulted in a dose-dependent inhibition of viable cell count and proliferation in both T98G and U87MG glioma, and BTSCs.
It is generally accepted that androgens induce epithelial proliferation in vivo when the cell count is below normal, and androgen administration following castration induces rapid prostate regrowth in the rat [ 23, 61].
Cell proliferation in culture was evaluated by counting cell numbers.
To assess cell migration and proliferation in the micro-wound, cell counts were averaged across the two center strips and to assess cell proliferation at the edge, cell counts from the green channel images were averaged across the four peripheral strips at the far left and right edges of the image.
In regions that appear homologous to the areas where gray matter reductions are evident in depressed humans (i.e., medial PFC, hippocampus), repeated stress results in dendritic atrophy and reductions in glial cell counts or proliferation in rodents (Banasr and Duman 2007; Czeh et al. 2005; McEwen and Magarinos 2001; Wellman 2001; Radley et al. 2008; Conrad et al. 1999).
To test for host sex differences in the rate of within-host proliferation, we counted spores in two groups at two different times after exposure (Experiment 3, Table 1).
In contrast, no group differences in mitogen-induced cytokine production or T-cell proliferation in vitro or in leukocyte subset counts were observed.
We found that regulating TGF-β1 had a significant effect on the proliferation rate of oral cancer cells determined using direct viable cell number counting, and cell proliferation in situ, detected using a BrdU FITC kit.
End points specific to this group included gross and histopathology, hematology, serum chemistry, and evaluations of bronchoalveolar lavage fluid for indications of cytotoxicity [lactate dehydrogenase (LDH) activity] and inflammation (total protein, total and differential nucleated cell counts), and splenocyte proliferation in response to mitogen in vitro.
