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They have been shown to exhibit two important properties: (1) proliferation in sufficient amounts to produce cell mass for therapeutic purposes and (2) differentiation into different cell types.
The effect of low oxygen tension on spiral ganglion SPCs not only resulted in enhancing proliferation in the cell amount and diameter of neurospheres, but also increased the protein expression of cyclin D1 and PCNA, while at the same time suppressing the p27 level.
Glycine starvation prolonged the G1 phase of the cell cycle and reduced proliferation, in part because sufficient amounts of this amino acid are required to support de novo purine biosynthesis in rapidly dividing cells.
The amount of proliferation in vector co-transfected cells therefore served as the baseline to assess the effects of p16 and p21 on PyLT-induced proliferation.
However, the limited OPC proliferation in vitro, which requires large amount of human donor tissue, and their potential immunogenicity might restrict their use in future clinical applications.
The difference in fluorescence between the two time points (24 h and 1 week) was calculated and considered the amount of proliferation in that time window.
Immobilization causes further reorganization of the synovial layer: decrease in synoviocyte proliferation and in the amount of synovial fluid [ 36– 36].
Based on the observation that the haltere imaginal disc has ∼3-fold fewer cells than the wing imaginal disc, Ubx is expected to regulate genes that reduce the amount of cell proliferation in the haltere.
Pac and zeo require higher levels of expression to support the proliferation in the presence of the lethal amount of drugs than others, which allowed us to obtain the transfectants with homogenously high levels of transgene expression.
Allogeneic UC-MSCs suppressed T cell proliferation in lupus patients by secreting large amounts of indoleamine 2,3-dioxygenase (IDO).
The study of Requena et al. [ 19] compared these parameters together with lymphocyte population and proliferation, in two groups infected with different amounts of parasites and a control group.
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