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Fig. 8 SeNPs@Am alters HepG2 cell biobehaviors via affecting the cell proliferation, cycle and apoptosis.
Increment in differentiation efficiency of the mesenchymal stem cell was obtained by using hydrogel to adjust the proliferation cycle of encapsulated cells to signal sensitive phase.
By changing the G′ value of hydrogels under encapsulation the cells, proliferation cycle of encapsulated mesenchymal stem cells was regulated to G1 phase and thus signal sensitivity were increased.
Although NP is known to be the most, at least one of the most, stable proteins encoded by −ssRNA virus during their proliferation cycle, significant structural shift can be observed in various stages among multiple virus species (Zhou et al., 2013).
As a typical negative-sense single-stranded RNA (−ssRNA) viruses, EBOV possess a nucleocapsid protein (NP) to facilitate genomic RNA encapsidation to form viral ribonucleoprotein complex (RNP) together with genome RNA and polymerase, which plays the most essential role in virus proliferation cycle.
That is, when the G′ value of the hydrogel was higher than 1.0 kPa, the cell proliferation was ceased and the proliferation cycle of cells was converged to G1 phase, whereas when the G′ value was below 1.0 kPa, cell proliferation proceeded.
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The maximum effect was observed on the 5th day of incubation (two proliferation cycles).
The number of cells remained unchanged after two or three proliferation cycles (20%, as compared with the control).
Consequently, we recommend avoiding direct use of embryogenic callus for transformation experiments and instead completing a number of preliminary proliferation cycles to increase the quantity of PEMs.
In vascular and other green plants, it is very difficult to obtain synchrony of proliferation cycles because of the abundant mitochondria and chloroplasts in the cell.
In accordance with a previous study, these results suggest that imatinib exerts its effect by arresting DNA replication and the cell-proliferation cycle [11].
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