Sentence examples for proliferation conditions from inspiring English sources

Cells were cultured in proliferation conditions and total RNA isolated using the RNeasy Mini Kit (Qiagen) according to manufacturers instructions.

For ATRX ChIP18, cells in three biological replicates cultured under proliferation conditions were fixed with 2 mM EGS (Pierce) for 45 min at room temperature in PBS.

As expected, we found O4-expressing cells displaying a branched OL morphology only under differentiation but not under proliferation conditions (Fig. 1b).

Firstly, the intact virtual epidermis was simulated for 100 iterations under default cell proliferation conditions (stem cell 1%, TA cell 2%).

The fact that CBF is a transcription factor which interacts with MyoD preferentially in proliferation conditions, led us to investigate whether it would be targeted to MyoD target genes to repress their transcription.

Under proliferation conditions, cell density was significantly greater with EGF or FGF2 alone than without any growth factors, suggesting that both factors exerted a pro-proliferative effect, with EGF being more potent than FGF2 (Fig. 4b).

A2B5+ NPCs treated with siRNA of TRPC5 hardly proliferated in the proliferation condition, although the expression level of TRPC5 in A2B5+ NPCs was not high compared with differentiated cell.

In addition, there was no significant difference between the predifferentiation condition prior to incubation in chondrogenic medium and the proliferation condition, which suggests that in situ chondrogenic differentiation of hASCs was induced by the TGF-β1 loaded nanoparticles.

There was no significant effect of siRNA on morphology or immunophenotype in the proliferation condition (Figure 6A).

Under the proliferation condition (i.e., in medium lacking osteogenic factors), the mineral content was sufficient to encourage ASCs toward osteogenic differentiation.

We found that edema development in NPHP8 RNAi) animals was faster and more severe under the increased proliferation condition, yet significantly diminished or even abolished under reduced or no proliferation, respectively.