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A2B5+ NPCs treated with siRNA of TRPC5 hardly proliferated in the proliferation condition, although the expression level of TRPC5 in A2B5+ NPCs was not high compared with differentiated cell.
In addition, there was no significant difference between the predifferentiation condition prior to incubation in chondrogenic medium and the proliferation condition, which suggests that in situ chondrogenic differentiation of hASCs was induced by the TGF-β1 loaded nanoparticles.
There was no significant effect of siRNA on morphology or immunophenotype in the proliferation condition (Figure 6A).
Under the proliferation condition (i.e., in medium lacking osteogenic factors), the mineral content was sufficient to encourage ASCs toward osteogenic differentiation.
We found that edema development in NPHP8 RNAi) animals was faster and more severe under the increased proliferation condition, yet significantly diminished or even abolished under reduced or no proliferation, respectively.
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Firstly, the intact virtual epidermis was simulated for 100 iterations under default cell proliferation conditions (stem cell 1%, TA cell 2%).
The fact that CBF is a transcription factor which interacts with MyoD preferentially in proliferation conditions, led us to investigate whether it would be targeted to MyoD target genes to repress their transcription.
Under proliferation conditions, cell density was significantly greater with EGF or FGF2 alone than without any growth factors, suggesting that both factors exerted a pro-proliferative effect, with EGF being more potent than FGF2 (Fig. 4b).
However, this was not obvious under default cell proliferation conditions even when the differentiation rules in the model were relaxed and the intact virtual epidermis was also simulated for 100 iterations (Table 1), suggesting psoriasis might be related to both cell proliferation and differentiation.
The cells were cultured under proliferation conditions in ITSFn medium supplemented with 20 ng/ml bFGF (Sigma), 2 ng/ml NT3 (R&D systems, Minneapolis, MN) and 20 ng/ml PDGF-AA (Sigma), a combination that was known to permit in vitro expansion of A2B5+ cells [13].
Our results showed that, in contrast to control cells in which histone H3 acetylation (a mark associated with transcription activation) on myogenin, cyclin D3 and p21 promoters increased in differentiation compared with proliferation conditions, histone H3 acetylation levels at these promoters did not vary in C2C12-CBFβ cells (Figure 5C).
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