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To understand the progressive enlargement of eGFP-KRASG12D-positive masses, we assessed cell proliferation by using an antibody that specifically labels the PCNA (proliferating cell nuclear antigen) protein, a specific marker for cells in S phase of the cell cycle.
We evaluated the ability of conditioned medium samples to induce cellular proliferation by using MTT assays [7].
We assayed cell proliferation by using BrdU incorporation assay.
Newer techniques include detection of antigens closely associated with proliferation by using immunohistochemistry (IHC).
CD19+/CD3 -/CD14- B cells were analyzed for their proliferation by using CFSE labeling.
We assayed cell proliferation by using direct cell count and BrdU incorporation assay.
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This study aimed to assess the prognostic value of a proliferation assay by using Ki-67 immunohistochemistry as compared with mitotic count scores.
In conclusion, proliferation assessment by using Ki-67LI and MS can distinguish subgroups of patients with luminal/HR+ BC with significantly different clinical outcomes.
Breast cancer intrinsic subtypes using both four- and six-marker immunohistochemical panels and proliferation assessed by using Ki-67 were determined in a large and homogeneous cohort of patients collected prospectively.
To determine the relationship and relative contribution of these two factors, we quantified caspase 3/7 activity and proliferation simultaneously by using high-content live cell imaging (see IncuCyte ZOOM description in the Methods section).
Bioactivity of vIL-10 in the fusion protein was tested with a cell-proliferation assay by using mouse MC-9 mast cells as described [ 18].
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