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(d) In vitro T-cell proliferation assay.
MTS cell proliferation assay was performed as described previously37.
(c) CCK8 cell proliferation assay for Hela cell.
Proliferation of BMMCs was determined using the CellTiter 96 Aqueous One Solution Cell proliferation Assay kit.
Cell count proliferation assay was performed using IncuCyte ZOOM system according to the manufacturer's protocol.
For proliferation assay, mice were received single pulse of BrdU (50 mg/kg; Sigma-Aldrich) injection.
Each proliferation assay detected the known radiation susceptibility gene, PRKDC.
Tumor proliferation was measured by WST proliferation assay.
Cell proliferation assay: PDLSCs (P3) were seeded in 96-well plates (Invitrogen) at 2 × 104 cells/well.
For in vivo proliferation assay, each T cell subset was obtained from CD45.1+ CD4+ OT-II mice.
After 72 h of culture, the cell proliferation was measured with a MTT cell proliferation assay kit (Cayman).
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