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HEK293T (dashed) and CG-4 (solid) cell proliferation as a function of increasing oleic acid (red = 50 µM, grey = 5 µM, black = 0 µM).
(A ) HEK293T (dashed) and CG-4 (solid) cell proliferation as a function of oleic acid or stearic acid treatment (red = treated, black = untreated).
This systems biology model offers a paradigm closely related to biological phenomena and highlights previously unexplored interactions of cell elongation, migration and proliferation as a function of ligand concentration, giving insight into key cellular mechanisms driving angiogenesis.
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Fig. 6 Cell proliferation result as a function of time in culture after dispensing at different positive pressures.
The cell-level model, in turn, provides other benefits – modeling cell behavior at a level where not all molecular details are known, and when an event (e.g., proliferation, migration as a function of growth factor) or sequence of events governs capillary growth.
It is defined as the same experimental-based equation for tip cells Ptip and stalk cells Pstalk (Equation 10), however stalk cell proliferation occurs deterministically whenever Pstalk is allowed, while the tip cell proliferation occurs probabilistically as a function of Dll4 (Table 3).
Cell proliferation is measured as a function of changing electrical impedance as per the manufacturer's instruction and analysed using RTCA software (Roche).
MTS and TUNEL assays were used to study cell proliferation and apoptosis as a function of salinomycin exposure in JLO-1, a putative HNSCC stem cell culture.
Human MSC proliferation was assessed as a function of metabolic activity using the WST-1 assay (Roche Applied Science, Indianapolis, IA, USA) [ 18].
Cell proliferation was measured as a function of 3- 4, 5-dimethylthiazolyl-2 -2, 5-dimethylthiazolyl-2 -2omide 5-dimethylthiazolyl-2 -2iferation 5-dimethylthiazolyl-2 -2the manufacturer's instructions.
Cell proliferation was studied as a function of the culture time and results are presented in Figure 5. Doubling the seeding time resulted in approximately 7-fold increase of cell number.
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