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Transfection of cells with scrambled human siRNA resulted in proliferation approximately equal to the controls.
For the experiments to assay contact inhibition of proliferation, approximately 1 × 103 cells/cm2 were plated.
The same keratinocyte-conditioned medium also induced a modest increase in fibroblast proliferation (approximately 1.8-fold).
Chemo-residual tumor cells resumed proliferation approximately 2 weeks post chemotherapy treatment.
Figure 2A illustrates that during basal proliferation approximately 20% of the cells were in S-phase.
However, a subpopulation (0.1 %) of chemo-resistant tumor cells persisted and resumed proliferation approximately 2 weeks after chemotherapy removal.
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Moreover, the bFGF gradient hydrogels increased SMC proliferation by approximately 41% and migration by approximately 15%, with cell migration observed in the direction of increased bFGF concentration, demonstrating control over cell mobility.
In particular, neither m7E3 at a concentration that reduced insulin-induced proliferation by approximately 80%, c7E3 at a concentration that reduced insulin-induced proliferation by approximately 55% nor eptifibatide at a concentration that inhibited insulin-induced proliferation by 83% had any discernible effect on levels of tyrosine phosphorylated ERK.
Although we recognize that post-pneumonectomy lung growth is a particularly robust example of tissue regeneration, the active proliferation of approximately 12% of all lung cells [10, 11], it does highlight the utility of in vivo labeling of regenerating tissues.
Therefore, to compare the aging effects on the growth kinetics and differentiation potential of young and aged ADSCs, we found that there was a significant decline in both the proliferation rate (approximately 13.3%) and osteo-differentiation potential in aged ADSC.
If the CC50 value is much higher than the concentration that can inhibit virus proliferation of approximately 20-fold, we believe that this inhibition to virus growth is specific and will subsequently measure the inhibition to the replication of viral genome, the expression of viral protein, and the production of mature virus.
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