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Our results demonstrate that meprin metalloproteases are important for cell differentiation and proliferation already during embryogenesis, predominantly by the activation of growth factors.
The demethylating agent 5-aza-2'-deoxycytidine arrest proliferation already at low concentrations in lymphoid cells, and demethylation could thus not be assessed.
As compared to a medium with 4 m M NH4Cl, CMX is more cytotoxic and inhibition of cell proliferation already occurs within 48 h.
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Balkwill et al. write that if genetic damage is the "match that lights the fire" of cancer, then inflammation is the "fuel that feeds the flames" and that inflammation affects both the survival and proliferation of already initiated cancer cells[38].
After 5 weeks of iBRAF* induction in 8-week-old animals (left panel in Figure 2A), the prostate gland appeared largely normal although moderate degree of aberrant proliferation was already evident (Figure 1E).
Note that in the [3H]Thymidine based suppression assays (Figure 10A), the low counts observed after titration of high numbers of expanded Tconv are not indicative of suppression per se, but rather a result of the very early proliferation of already primed Tconv cells in the co-culture.
It was therefore unknown whether extensive proliferation was already present.
However, in these conditions the extent of IGF1-induced proliferation was already modest.
Indeed, a CPC phenotype with increased proliferation was already observed in the small intestine by day 5 (Fig EV5).
Downregulation of H1.2 indicates that the rate of cell proliferation has already decreased in the preclinical stages of the syndrome.
This was not only seen in minimal medium (1% FCS) but also in optimal medium (10% FCS) in which proliferation was already quite fast.
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