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Similarly, GLI1 overexpression did not augment proliferation, again in contrast to the EAC cells [ 25].
After several rounds of clonal expansion, cells arrest proliferation again and undergo terminal adipocyte differentiation.
Later, when the environmental conditions improve, spores germinate to spawn haploid cells which then undergo normal asexual proliferation again.
Tumor cells may stop cell division processes after dissemination but may persist in a quiescent status until environmental conditions provide appropriate signals to start proliferation again.
The accumulation of oligoclonal T-cell populations appears to be more the consequence of a prolonged survival than increased proliferation, again consistent with the concept of cellular senescence.
On the other hand, RANK expression was associated with activation of immune response and proliferation, again consistent with the high expression of RANK in the poorly differentiated, triple-negative tumors in which immune-related pathways are emerging as important targets in this tumor subtype [ 23].
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Schultze et al. [20] cultivated CD40-activated B cells for up to 65 days without observing B cell differentiation or a decrease in proliferation, but again EBV infection was found in an unspecified proportion of the cultures.
The cell proliferation was again assessed by the XTT assay.
Effects of SC-236 on cell proliferation were again evident (33% mean reduction in proliferation, p = 0.001).
These findings were even more pronounced when using citrullinated fibrinogen as stimulating antigen: 10/15 (67%) RA patients were positive for T-cell proliferation and again only 1/14 control patients was clearly positive (P < 0.0001).
As for non-adherent cells, ASS-positive cells demonstrated almost total resistance to the anti-proliferative effects of ADI-PEG20, whereas a relative decrease in proliferation was again observed in ASS-deficient cells following ADI-PEG20 treatment.
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