Exact(16)
Cultured in proliferating medium, human mesenchymal stem cells (hMSCs) encapsulated in the fibers showed higher proliferation rate than those seeded on the scaffold.
Single clones maintained in proliferating medium [5] were left for 10 days and examined daily at phase contrast microscope.
Cells were expanded in proliferating medium in a humidified incubator at 36°C and 5% CO2.
Emasculated florets were pollinated several times over two days and embryos were removed after nine days and placed on a shoot proliferating medium CR7 [ 36].
In proliferating medium, 98 ± 1% of Ct-SC and 97 ± 2% STX-SC expressed p75 (Supplementary Fig. 1A and B) and S100 (Supplementary Fig. 1C and D), antigens expressed by all Schwann cell stages.
Embryos were transferred to proliferating medium where secondary embryogenesis was observed and after repetitive subcultures the existing embryos could proliferate by producing embryogenic masses ("ms" in Figure 1J) from which new embryos were originated (arrow in Figure 1J).
Similar(44)
Two weeks after the selection in G418, drug-resistant clones were isolated and allowed to proliferate in medium containing G418.
These cells exhibited a fibroblastlike, normal morphology; were anchorage-dependent; and were unable to proliferate in medium with reduced serum concentrations.
Ba/F3-gp130 cells did not proliferate in medium without stimuli however, H11 stimulated these cells in a at dose dependent manner.
For embryo induction, one gram of friable callus proliferating on ENT medium was transferred to a 250-mL bottle containing 50 mL of expression medium (EXP).
This indicates that N2 medium induces both salivary gland epithelium and mesenchyme to form spheres, and a majority of cells proliferating in N2 medium were epithelial cells.
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