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With the increasing number of genome and metagenome projects, sequence databases have grown exponentially.
Functional assignment in the enolase superfamily provides a paradigm for the challenges faced in assigning functions to unknown enzymes discovered in genome projects: sequence homology alone is insufficient to transfer functional annotations from previously characterized members.
First of all, in nextgen sequencing projects performed to a high depth of coverage (>20-fold is common in 454 projects) sequence gaps between contigs are rare due to the relatively unbiased libraries generated by these new technologies; fragmentation into contigs is largely due to the presence of repeats.
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Our WG-FAST pipeline is able to phylogenetically genotype isolates from single isolate sequencing projects, low coverage sequencing projects, or from complex samples with variable coverage, such as metagenomics projects sequenced from human samples.
Despite these methodological advances in large-scale projects, sequencing these AT-rich and complex mtDNAs remains challenging, especially for smaller scale studies.
However, programs used to genotype the current phase 1 release of 1000 Genomes Projects (sequencing based) are unable to genotype sites with more than two alleles.
Briefly, insert ends were dideoxi-sequenced with m13-pUC18 primers and aligned against the L. infantum genome project sequence in General Feature Format (GFF) deposited in a GBrowse database.
Looking inward, he felt "a disconcerting mixture of nostalgia, regret, claustrophobia, emptiness, and fear"; looking forward, he saw only "a projected sequence of accomplishments stretching through the future to retirement, decline, and death".
His projected sequence began with "The Ice-Shirt" (1990), wherein he turned the searchlight of his imagination on the 10th century, when the first explorers ventured toward the New World.
In a genome project, sequence annotation is a crucial step.
Little Skate Genome Project sequence and annotations are collected under GenBank BioProject 60893.
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