The Ngai Lab BRAIN Initiative Project: Cell Type Classification in the Brain.
Change in water content within cells (cellular dehydration) was measured based on observed changes to projected cell area during freezing.
At lower densities of immobilized peptide, decreases in both cell attachment and projected cell area were more pronounced.
The muscle fibres of vertebrates and higher invertebrates, on the other hand, have no projecting cell body.
The response of the cells was determined in the form of projected cell area measurements by high-resolution observation before, during, and after fluid shear application.
The alternative method is to project cell volumes along Lagrangian trajectories as far forward or backward in time as desired, and then to remap the resulting density distribution onto some target mesh.
When an as-prepared sample was dried in a vacuum at room temperature, the tetragonal modification was transformed into the monoclinic one with the projected cell dimensions of a = 1.75, b = 1.36 nm and γ = 110°.
Projected cell area was measured using ImageJ.
As the media pH increased toward neutral pH, cells eventually began to shrink and thus the projected cell area decreased.
For our needs the estimates were adequate, particularly since projected cell images (Fig. 2B) exhibited consistently high circularity.
