Axonal projection types were determined using a cluster analysis in the 2D parameter space of the axon barrel ratio and the axon home column ratio as defined above.
Discriminant variables were determined using VIP (variable importance in the projection), an appropriate quantitative statistical parameter ranking the descriptors according to their ability to discriminate different doses.
The perikaryal size of PCs and DCN projection neurons was determined using ImageJ on 1-µm-thick confocal images captured under a 63x objective.
Image stacks were processed to maximum projections, and dendritic spine density was determined using ImageJ.
The collimator and acquisition parameters in the astigmatic reconstruction formula, which include focal lengths, radius of rotation, and mechanical shifts, are often partly unknown and can be determined using the projections of a point source at various projection angles.
Finally, this rotation allows the jaw positions to be determined using vertical and horizontal projection profiles.
Therefore, the focal projections were determined at the light microscope using a 1.6× objective and directly outlined using the Neurolucida software (MicroBrightField, Inc .. Adjacent patches of dense fibres (visible at 1.6×) were often surrounded by less dense fibre labelling (visible at 4×).
The projections are determined by training data using both distorted and undistorted data and applying the oriented principle component analysis (OPCA).
The factor scores obtained for these samples were determined by projection, using factor loads calculated from day 0 data.
First, a projection constant A was determined by using the known stars detected in the image.
