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Projections were collected at 128 different angles, equally spaced, over 360°, in 128×128 matrices (3.30 × 3.30 mm2 per detector element).
Projections were collected at 128 different angles, equally spaced, over 360°, in 128 × 128 matrices (0.33 × 0.33 cm2 per detector element).
We set 2003 as the baseline year for our projection because the default data for the new LiST projections were collected to respond closely to the year 2003.
In total, 1,800 projections were collected at a resolution of 6.93 μm/pixel.
2000 projections were collected at an exposure time of 9 14 s for each projection.
3,142 projections were collected for each scan and reconstructed using a modified Feldkamp back-projection algorithm [ 41] in CT Pro (Nikon Metrology, Tring, UK).
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Using the transmission electron microscope, these projections are collected at a number of different orientations, typically by tilting the sample about a fixed tilt axis [4], while other dual axis tilting schemes also exist [5].
To overcome the ill-posedness and get high resolution and robust reconstructed images, a large measured fluorescent data set over the surface of the small animal from 360° projections is collected by a high performance charge-coupled device (CCD) based non-contact full angle FMT imaging system [ 12].
Two dimensional transmission images (projection images) were collected and the projection images were corrected for the dark signal from the detector (dark signal correction) and flat signal (flat-field correction) for imperfections from the monochromator and scintillator screens.
Projection images were collected at 1 1.5 ° angle steps for 360 ° full rotation to check for self-absorption effects (Supporting Information).
Dose fractionated data (3 5 frames per projection image) were collected using Digital Micrograph (Gatan, Pleasanton, USA) at a nominal magnification of 34,000× (corresponding to a pixel size of 0.66 nm) in the Polara or at 33,000× (corresponding to a pixel size of 0.42 nm) in the Krios.
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