The idea of Unlimited is that galleries can present work that won't physically fit within the confines of the regular Basel booths: large-scale sculptures, video projections, site-specific pieces, performance art and installations are all included.
The NAc, the terminal projection site of the mesolimbic dopamine system, is speculated to be critical to the etiology of schizophrenia and critical to reinforcing the properties of drug abuse (Chambers et al, 2001).
In the spinal trigeminal nucleus (medullary dorsal horn), which is the central projection site of most of nociceptive trigeminal afferents, CGRP immunoreactivity is found preferably in the superficial layers I-II [23].
Since leucokinin release is likely to occur at peripheral release sites on body wall muscles [67], it is possible that a synaptic input region is located along the VL tract, the only projection site of abdominal leucokinin neurons within the CNS neuropil.
To determine the projection site of GRP-immunoreactive axons, double-immunofluorescence staining of GRP (1∶5,000 dilution) and nNOS (A-11; mouse monoclonal antibody, Santa Cruz Biotechnology, Santa Cruz, CA) (1∶8,000 dilution), a marker protein for neurons in the SPN, was performed as described previously [20].
The projection site and converged glomerular structure of OSNs expressing the EGFP reporter suggest proper expression of the MOR42-3 transgene.
We found that each population of neurons (with the same projection site) included neurons in both VTA and SNc.
Because MOR42-3 and MOR42-1, members of the MOR42 subfamily, share 97% amino acid similarity in their coding sequences, the axonal projection site of MOR42-3-expressing OSNs was expected to be close to that of MOR42-1.
We found many fewer neurons in the SNr when we labeled dopamine neurons only, based on their projection site (i.e. in cases where we did not allow for spread to inputs).
Given that injecting pseudotyped rabies virus alone resulted in very few infections (Watabe-Uchida et al., 2012), these results indicate that the injection of AAV5-FLEX-TVA into a dopamine projection site allowed us to restrict infection of rabies virus to dopamine neurons in a projection specific manner.
To do this, we injected a helper virus that expresses TVA under the control of Cre recombinase (AAV5-FLEX-TVA) into a dopamine projection site and co-injected a virus bearing blue fluorescent protein (AAV1-CA-BFP) to mark this site.
