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For the image comparison, we generated en face averaged projection images of the photoreceptor layer (PRL) with a projection thickness of 52 µm (21 pixels).
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To quantify neurogranin and GlyT2-eGFP staining intensities at Golgi cell bodies, z-stacks containing the somata were projected and averaged (z-projection thickness: 6.8 µm).
(Z-projection thickness: 4.6 µm, scale bar: 10 µm).
(E2 ) Higher magnification of iNC axon (arrows) co-stained for EYFP and GAD65-77 (Z-projection thickness: 2.4 µm).
(E ) iNC boutons (green) transfected with AAV-flox-EYFP in GlyT2-cre mice are stained for GAD65-67 (rE1, E1, Z-projection thickness: 8.2 µm).
Right: GoC patched and filled with Neurobiotin (green) in GlyT2-Cre mice surrounded by transfected axons (red) (Z-projection thickness: 36.4 μm; Sagittal view).
(D ) Merged confocal image (Z-projection thickness: 12.2 μm) showing iNC axons in GAD-cre mice injected with AAV-flox-EYFP (green) are co-stained for GlyT2 (red) (D1 ).
Statistical analysis was performed on the overall projection, bony thickness, and soft tissue thickness.
Geographic location (Gauß-Krüger projection), clay thickness to 1 m soil depth (CTH), distance to water table (DWT) (Table 1), available from other data sources, were used as predictor variables.
Surface projections of cortical thickness differences between ex-preterm and control adolescents are shown in Figures 1, 2, and 3. Thinner cortex was observed in widespread regions in ex-preterm adolescents compared with controls (Fig. 1 a ).
From a geometric point of view, the measured shell thickness in projection of a uniform shell with thickness a varies as (frac{a}{cos theta }).
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