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Given that injecting pseudotyped rabies virus alone resulted in very few infections (Watabe-Uchida et al., 2012), these results indicate that the injection of AAV5-FLEX-TVA into a dopamine projection site allowed us to restrict infection of rabies virus to dopamine neurons in a projection specific manner.
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Lastly, we predicted that cortical networks involving frontal-parietal circuits and insular and opercular regions would become activated similarly for both pacing conditions with the latter two possibly involved in sensory-to-motor projections specific to auditory-motor synchronization.
Comparison of the percentage of inputs from selected regions across populations of projection-specific dopamine neurons.
Labeling projection-specific dopamine neurons and their monosynaptic inputs throughout the brain with rabies-GFP.
(A ) A schematic of the injections used to label projection-specific populations of dopamine neurons.
10.7554/eLife.10032.014 Figure 5. Correlation between projection-specific populations of dopamine neurons.
10.7554/eLife.10032.006 Figure 3. Distribution of monosynaptic inputs to projection-specific populations of dopamine neurons throughout the brain.
In the present study, we found that most populations of projection-specific dopamine neurons are distributed both in VTA and SNc.
(G ) A schematic of the injections used to label the inputs of projection-specific populations of dopamine neurons throughout the brain.
DOI: http://dx.doi.org/10.7554/eLife.10032.006 10.7554/eLiFigure3 figureigure 3—figure supplement 1. Distribution of projection-specific populations of dopamine neurons within the midbrain.
DOI: http://dx.doi.org/10.7554/eLife.10032.007 10.7554/eLiFigure3 figureigure 3—figure supplement 2. Distribution of projection-specific populations of dopamine neurons within the midbrain: Maximum Intensity Projection.
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