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In the spinal trigeminal nucleus (medullary dorsal horn), which is the central projection site of most of nociceptive trigeminal afferents, CGRP immunoreactivity is found preferably in the superficial layers I-II [23].
To determine the projection site of GRP-immunoreactive axons, double-immunofluorescence staining of GRP (1∶5,000 dilution) and nNOS (A-11; mouse monoclonal antibody, Santa Cruz Biotechnology, Santa Cruz, CA) (1∶8,000 dilution), a marker protein for neurons in the SPN, was performed as described previously [20].
Since leucokinin release is likely to occur at peripheral release sites on body wall muscles [67], it is possible that a synaptic input region is located along the VL tract, the only projection site of abdominal leucokinin neurons within the CNS neuropil.
We found that each population of neurons (with the same projection site) included neurons in both VTA and SNc.
The projection site and converged glomerular structure of OSNs expressing the EGFP reporter suggest proper expression of the MOR42-3 transgene.
It remains to be examined whether VTA dopamine neurons with a specific projection site receive a different set of monosynaptic inputs compared to SNc dopamine neurons that project to the same site (i.e., VTA → DS neurons vs SNc → DS neurons).
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DRGs corresponding to the lumbar spinal cord levels L1/L2/L3 were selected as the main projection sites of primary afferent fibers innervating the femur [39], [40].
10.7554/eLife.10032.018 Figure 8. Reciprocity of connection between dopamine neurons and neurons at their projection sites.
To verify this, we injected AAV5-FLEX-TVA into one of the projection sites, the DS.
Remaining panels depict the following projection sites: AHN, BNST, LHA, MeA, PAG.
The premise is that PD may affect dopamine projection sites that encode aspects of stimulus value.
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