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Particles from classes that showed the same projection average (denoted as 1, 2 and 3 in Figures S2B and Figure S3B) were combined (summarized in Table S1).
To demonstrate retinal and choroidal OCA, en-face projection (average over retina/choroid thickness) images of the Doppler shift squared (square of Eqs. (7) or (10)) are obtained.
Quantitative information on the size and distribution of oligomers was obtained by measuring the diameter of circular averages generated for each 2D projection average.
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After image classification, several projection averages showed particles with defined, but variable shapes with a length of ∼200 Å and a width of ∼100 Å (Figure 1D and Figure S1).
(bottom) Projection averages were removed for clarity.
(G ) Projection averages of AKAP18γ PKAholo complex assembled an RIIα ZeChimera construct.
Projections from the density map were calculated at angular intervals of 2º and cross-correlated with the respective projection averages.
2D reference-free alignment and classification was performed using SPIDER (Frank et al., 1996) to generate projection averages.
(F ) Projection averages of the AKAP18γ PKAholo complexes assembled with a truncated RIIα Δ44 86 construct using ISAC (Yang et al., 2012 ).
(F ) Projection averages of the AKAP18γ PKAholo complex classified into distinct conformations using ISAC (Yang et al., 2012 ).
A large single particle data set of fractionated H104K-HSPB5/αLac H104K-HSPB5/αLacecomplexnd 2D projection averages wase determined as above.
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