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Four multiparous Holstein cows, fitted with large diameter plastisol rumen cannulae between 6 and 8 weeks prior to their projected calving date, were assigned in a 4 × 4 Latin square design experiment between 2 and 4 weeks post-partum.
Four multiparous Holstein cows, fitted with large diameter rumen cannulae between 6 and 8 weeks prior to their projected calving date, were assigned in a 4 × 4 Latin square design experiment between 2 and 4 weeks post-partum.
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Six multiparous and 6 primiparous Holstein cows equipped with ruminal cannula were assigned to 6 blocks of 2 cows each according to parity, projected milk production at 305 d, and expected calving date.
In addition, homozygous deletion cows had the latest (P < 0.05) Julian calving date.
Cows with deletion haplotypes had a lesser (P < 0.05) calving percentage, and the latest (P < 0.05) Julian calving date when compared with cows having other SNP haplotypes.
This data was checked for inconsistencies in parity, breed, calving date and age at calving.
For instance, records with culling date earlier than calving date were excluded.
For each cow, information was obtained on calving date in 1997, parity, date of removal, disease date and disease code.
These contained information about age and pedigree of heifer, insemination dates and pedigree of bull, calving date, single or twin birth, calving ease (1 = easy, 2 = normal, 3 = difficult), attitude of calf, status of cow after calving and retained placenta.
Information on herd, calving date and lactation number was also collected for each lactation.
The model included the effect of GH and IGF-I genotypes as fixed effects and calving date as a covariate.
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