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The retrospective nature of the study design reflected the need to analyse multiple clinical variables and evaluate different types of outcomes as well as their progression over several years.
We are not aware of any known single nervous disease entity of adult pigs described in the veterinary literature that could present with the combination of signs described, without marked progression over several months and without obvious histopathological changes.
Criteria for diagnosis of HD were: 1) onset before 25 years of age; 2) unilateral or bilateral weakness of the distal upper limbs, accompanied by distal amyotrophy of the hand and forearm; 3) unaffected lower limbs; 4) unilateral or asymmetric; and 5) stabilization of the disease after an initial slow progression over several years [ 7, 8].
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This may be because the continuously erupting mouse incisor ameloblast progresses from the secretory stage to the final maturation stage in a matter of weeks, whereas this progression occurs over several years for human teeth.
This work supports the incorporation into mathematical models of disease of the clinical intuition that the history of disease in an individual matters, and represents an advance over several prior simulation models that assume a constant rate of disease progression.
"The screens are narrative; they show a progression of one battle over several days," Mr. Longhi said.
Despite progression of chromatographic techniques over several decades, such alternatives to traditional planar silica gel stationary phases have not been fully evaluated.
We observed tumour shrinkage in several of the KC PTEN mice following rapamycin treatment (figure 1C,D), and even in those KC PTEN tumours that did not regress, little progression was observed even over several weeks of follow-up.
Maintaining IOP ≤18 mmHg has been associated with less disease progression and increased stability of visual fields over several years of patient follow-up.
To confirm apoptotic cell death, we used the sensitive CICR (Caspase-Independent Cytochrome c Release) assay that prevents late-stage caspase-dependent apoptosis progression, thus allowing apoptotic cells to accumulate over several days.
This transcriptional progression (the ' Hox clock', Duboule, 1994) thus extends over several days.
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