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The reverse transcriptase was primed using an oligo dT primer and so the cDNAs will begin at the 3' end and will terminate randomly at variable sites as the enzyme progresses to the 5' end of the mRNA template.
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I occasionally run across YouTube videos that get hung up at a certain point and won't progress to the end of the clip.
But for one representative example, the loud twofold proposal of C major by the full orchestra and its quiet contradiction by the strings (at the end of the second movement) did not feel like two opposing ideas, or plea and demurral, but rather went by, loud-soft-loud-soft, as part of an emotionally neutral and rhythmically almost uninflected progress to the end.
It gave gamers 30 minutes to try to solve the puzzle and progress to the end of a brief mission wrought with zombies.
After the pre-action perception trials, participants walked down an 8 m walkway, stepped over a single obstacle (placed at 4 m), and progressed to the end of the walkway.
It gradually progresses to the point where end-to-end business processes can be automated, freeing up humans to make higher-level decisions on design and strategy.
"The grounds will be hard and we will need to move the ball around and score some tries if we are to progress to the upper end of the positions at the end of the competition.
During the last larval stage of development, nuclei that have progressed to the proximal end of each gonad arm cellularize and become sperm.
Factors that may protect an individual from progressing to the AD end of the spectrum of possible change as they age are legion.
That potential move is more likely to progress at the end of the season, with the Dutch defender's club reluctant sellers.
This study reports students' progress up to the end of year 2, including performance in the year 1 and year 2 summative examinations.
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