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The column was 30QC2/AC-5 30QC2/AC-5illary column (30 m × 0.32 mm) with the following conditions: column temperature: 180–280 °C; programmed increase, 3 °C/min; carrier gas: N2 (1 mL/min); injection and detector temperature: 250 °C; injection volume: 4 μL, split ratio: 1/50.
30QC2/AC-5 30QC2/AC-5illary column with the following conditions: column temperature: 180–280 °C; programmed increase: 3 °C/min; carrier gas: N2 (1.0 mL/min); injector and detector temperature: 250 °C; injection volume: 4 μL; and split ratio: 1/50.
The mechanisms through which the programmed increase in miR-483-3p miR-483-3p miR-483-3punknown.
The mechanisms underlying the programmed increase in HMG-CoA reductase are unknown; however, it is possible that epigenetic mechanisms are involved.
One μL aliquots of the extracts were injected splitless at 50°C (1 minute), followed by a programmed increase of oven temperature to 300°C at a rate of 10°C/min; helium was used as the carrier gas. 100 ng of n-octadecane was added as an internal standard to each sample.
Thorn et al. demonstrated in NHP that in utero exposure to HF diet-induced insulin resistance resulted in a programmed increase in hepatic triglycerides and upregulation of hepatic de novo lipid synthesis and inflammatory pathways, despite postweaning consumption of a healthy chow diet [ 40].
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