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Many viruses use programmed −1 ribosomal frameshifting to ensure the correct ratio of viral structural to enzymatic proteins.
These changes were also found to inhibit peptidyltransferase activity, stimulating programmed −1 ribosomal frameshifting and promoting virus propagation defects.
Introduction Machine learning is an emerging technique that enables computers to learn from data without been explicitly programmed [1], in the medical field has been used for classification and prediction analysis in both supervised and supervised fashion [2].
Although G36U1 pairing does not normally occur in cognate codon:anticodon interactions, this has been posited to occur at the P-site in Ty1 promoted programmed +1 ribosomal frameshifting [36].
Two of the major recoding mechanisms are programmed −1 ribosomal frameshifting (PRF) and stop codon readthrough.
The Programmed Ribosomal Frameshift Database (PRFdb) provides an interface to help researchers identify potential programmed -1 ribosomal frameshift (-1 PRF) signals in eukaryotic genes or sequences of interest.
Programmed −1 and +1 frameshifting test reporters were constructed by inserting a frameshift signal, L-A or Ty1 respectively, between the Renilla and firefly genes such that firefly luciferase can only be produced in the event of a frameshift.
Programmed -1 Ribosomal Frameshifting (-1 PRF) was first discovered in RNA viruses where it enables viral genomes to encode multiple peptides from a single mRNA [ 2].
Programmed −1 ribosomal frameshifting (PRF) and stop codon readthrough are two translational recoding mechanisms utilized by some RNA viruses to express their structural and enzymatic proteins at a defined ratio.
Program 1 concluded on Saturday; Program 2 continues through tomorrow.
Sunday at 2 and 6 p.m. (Program 1), and 4 and 8 p.m. (Program 2).
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