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For the first time, in fact, I've included a few kitchen implements to lift the hearts of your favorite cooks, including a programmable thermal coffeepot that, wonder of wonders, actually works.
The programmable thermal coffee maker from KRUPS is notable for the fact that it actually works, providing the taste of freshly brewed coffee even when it's sipped at different hours.
A programmable thermal management interface circuit for PowerPC systems has been designed, implemented, and tested for the Integrated Thermal Management (ITEM) System [1].
PCR was performed on a PTC-100 programmable thermal controller (MJ Research).
Polymerase chain reactions (PCR) were run in a programmable thermal cycler (MJmini, Bio-Rad).
PCR amplification of molecular markers was performed on a PTC-100 programmable thermal controller (MJ Research, USA).
The PCR conditions were as follows, initial denaturation at 94°C for 7 min, followed by 40 cycles denaturation at 94°C for 1 min, annealing at 37°C for 1 min and extension for 2 min at 72°C, followed by a final extension for 7 min at 72°C, using a programmable thermal cycler (Eppendorf).
PCR was carried out using Taq DNA polymerase (Qiagen, Germany) in a PTC-200TM Programmable Thermal Controller (BioRad, USA).
The PCR amplifications were performed using ampli-Taq (Applied Biosystems, Foster City, CA) and a programmable thermal controller Mx3000P QPCR system (Stratagene, La Jolla, CA).
For X-ray measurements, lipid samples (∼20 µl) were deposited between two thin mica windows and mounted on a programmable thermal stage (Linkam, UK).
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The reaction was initiated at 50°C for 15 min (RT) followed by denaturation at 97°C for 5 min. Then PCR was performed using a variable number of cycles of amplification that were defined by denaturation at 96°C for 1.5 min, annealing at 55°C for 1.5 min and extension at 72°C for 3 min (PTC-100, Programmable™ Thermal Controller, MJ Research, Inc., Waltham, MA).
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