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For the hybrid progeny, the same individuals were employed as parental oysters as for the within site ones, crossing French males with Danish females.
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(A ) Colorimetric WISH was performed on control, chd4 and p53 RNAi animals for smedwi-1 (stem cell marker), prog-1 (early progeny) and AGAT-1 (late progeny) from the same cohort of worms used for RNA-seq analysis.
In case of inconsistency, the progeny was removed, except when at least two progeny from the same sire were found incompatible with their sire.
Skewed inheritance of segregating CNVs were assessed using a Fischer's exact test comparing the observed number of progeny with an event to the expected number of progeny with the same event assuming a 1 1 Mendelian inheritance at each locus in the genome.
Because the selfed progeny on these plants can inherit the leaky S-allele through either the pollen and the ovule, the selfed progeny are more self-fertile (on average) than the outcrossed progeny from the same plant.
Subsequent F1 progeny were intercrossed and plasma HDL cholesterol was measured in the F2 progeny under the same conditions as the founder.
These measurements were collected on site #1 for 254 and 244 progeny in 2006 and 2007, respectively and on site #2 for 155 and 142 progeny for the same years (differences in sample size between years was due to mortality).
A phenotype of a = 0 corresponds to symmetric damage distribution (both progeny get the same amount of damage), whereas a = 1 corresponds to fully asymmetric distribution (one progeny gets all the damage, and the other none).
This explains, in part, the genetic variety seen among the progeny of the same pair of parents.
Matthew also indicated the possibility of developing new species: "the progeny of the same parents, under great difference of circumstance, might, in several generations, even become distinct species, incapable of co-reproduction".
Then her progeny does the same.
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