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Further genotyping of 48 of its selfed progeny identified a plant (designated 'WA8059') that was homozygous for 243 of the 251 loci (97% RPG).
Linkage mapping in the [A/JxAKR/J]F2 progeny identified a novel gene effect located on chromosome 8 and termed Carg3 (LO D= 3.95) and confirmed the chromosome 11 locus (Carg4, LOD = 4.59) (Figure 6).
Non-conditional mutations that inactivate genes can be used to study essential requirements, but such mutations must be maintained in heterozygotes and homozygous mutant progeny identified among progeny that vary in genotype.
PCR and sequence analysis of mutant progeny identified the precise deletion of yellow in the progeny of one founder.
Genotypes of mutant Δ rca-1 × Δ cre-1 progeny identified by PCR and 1.5% (w / v ) agarose gel electrophoresis.
Progeny identified as homozygous for the inbred ARC-f and ARC-r haplotype were tested for fusibility against allocompatible inbred lines.
Similar(48)
After backcrossing NILs to B73, selfing the resultant progeny, identifying recombinant haplotypes, and testing homozygous recombinant progeny lines, effects were resolved to a region of ∼10 Mb on chromosome 9 in both NIL families (CML277: 102,469,299–109,910,100, CML333: 99,948,772–109,910,100, RefGenV1).
In one, termed synthetic genetic array (SGA), double mutants are generated by mating the query mutant haploid strain to a panel of yeast knockout mutant strains; unviable double-mutant meiotic progeny identifies synthetic lethal relationships (Tong and Boone, 2005).
A total of 241 STRs were positioned at loci that were uniquely identified, and a total of 1748 STRs were positioned at loci that were each redundantly identified (e.g., two STRs with the exact same series of genotypes across the 44 F2 progeny identify the same locus).
Five samples of the Pre-Rif belonged to progenies identified as hexaploid wheat (BW type, see above) and were excluded from the genetic analysis.
Only 29% of all QTLs detected with cross D from 500 progeny were identified from 250 progeny and PPVE values were approximately twice as large as those obtained with 500 progeny.
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CEO of Professional Science Editing for Scientists @ prosciediting.com