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These segregations suggested that the mutated alleles, and more especially nof1-2, were transmitted to the progeny at a lower frequency than the wild-type allele.
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Thus, ER+ breast tumors also produced senescent progeny in vivo, but at a lower rate.
In all cases, early-maturing progeny produced a lower grain number per panicle and lower grain yield (Table 4).
Indeed, co-infected cells may produce recombinant viral progeny at levels lower than mutation rates in an intra-patient environment (Neher and Leitner, 2010).
In an unchanging environment, sexual reproduction of well-adapted genotypes is evolutionarily costly, meaning that most sexual progeny will have a lower fitness than the successful parent.
Moreover, CD133+ Ovarian cancer cell lines have been very recently shown to exhibit enhanced resistance to platinum-based therapy, and to form more aggressive tumor xenografts at a lower inoculum than their CD133- progeny [ 27, 28].
However, the presence of the tandem duplication event (scored with a dominant PCR marker) was detected in only 50% of the F3 progeny (the tandem duplication was present in 40 plants and absent in 40 plants), indicating that this locus was transmitted at a lower frequency than expected.
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Thus, ESC differentiated in our assay generated both myeloid and erythroid colonies, albeit at a lower efficiency as compared with CB. Cell suspensions derived from the clonal cultures of ES34+ were then analyzed by FACS (ES34− numbers were not sufficient to allow for FACS analysis) and compared to the progeny of CB34+ cells.
Then continue at a lower heat.
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