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A current focus in the field is to identify new molecules that control formation and function of the regeneration blastema, a mass of proliferative mesenchyme that emerges after limb or fin amputation and serves as progenitor tissue for lost structures.
The loss of cardiac tissue when BT10 is added early is likely due to loss of cardiac progenitor tissue, as seen with excess RA.
Immortalized leiomyoma and myometrial cells retained the molecular characteristics of their progenitor tissue.
Consequently, it is possible that our results with this cell line are biased by the overlap of A673 cells with neural progenitor tissue, which are known to harbor pluripotent stem cells [ 37].
Versican (3·05 ± 0·79-fold, P < 0·05), CYP26A1 (19·16 ± 3·98-fold, P < 0·05) and TGF-β3 (2·6 ± 1·02-fold, P < 0·05) demonstrated increased gene expression in leiomyoma cells, similar to the primary cultures and progenitor tissue.
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This correlates with defective coronary vascularization, and specific deletion of Ino80 in the two major coronary progenitor tissues-sinus venosus and endocardium-causes intermediate phenotypes.
To develop a rapid biomarker method for confirming that leiomyoma and myometrium primary cultures maintain the molecular phenotype of the progenitor tissues.
These methods tend to utilize cells and/or progenitor tissues in two- and three-dimensional culture systems, which can be combined to form seemingly functional kidney-like tissue, as judged by immunohistochemistry, gene expression, and transport capacity.
In addition to these ubiquitous progenitors, tissue fibroblasts have also been proposed to arise from circulating bone marrow cells or, in specific organs, from epithelial-mesenchymal transition (EMT) (see reviews [ 23, 24]); the controversial evidence supporting these claims will be discussed below.
Somatic progenitors sustain tissue self-renewal while suppressing premature differentiation.
Organ-specific stem cells are the natural progenitors in tissue regeneration and possess plasticity to differentiate into specialized cells in adult tissues.
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