Application of the µ-opioid receptor agonist, DAMGO (500 nM, bath), to the in vitro rhythmically-active medullary slice preparation caused a profound depression of respiratory activity (Fig 1A).
The changes in the transcriptome that occurs between 500 and 700 d° may largely represent a preparation for the profound structural changes that take place, particularly in the sheath when mineralisation commences, ultimately to form the chordacentrae.
Great care must be taken when comparing between studies because differences in sample collection and library preparation can have profound effects upon the small RNA profiles observed and the proportion of reads mapping to Y RNAs or exogenous small RNAs.
The preparation process had a profound effect on the morphology and particle size of the materials.
By the end of her preparation, Sarah had gained profound insight into herself and her past, and her relationships with her husband and children had greatly improved.
DNA preparation methods have a particularly profound effect in our study, implying that samples prepared with different protocols are not suitable for comparative metagenomics.
To the extent that revelation is identified with a profound and transforming personal experience, the spiritual preparation of the subject by prayer and asceticism is stressed.
The main drawback of NASBA is that special care is needed in sample preparation and detection, due to the profound susceptibility of RNA to degradation.
Completing the TransRockies was a marvelous experience, but my personal take is that the journey - including the arduous preparation required - was even more profound than the actual event.
The data suggest that perturbed endometrial preparation prior to conception will have profound consequences on embryo-maternal interactions in pregnancy.
