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Mosquito samples for quantitative real-time PCR (qRT-PCR) profiling were prepared from ten-fifteen individuals.
Samples for metabolite profiling were prepared as described in [48].
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CT26 oligo dT -isolated RNA foligo dT -isolated pRNAiling was prepared in triplicate.
In addition, differential graphs illustrating MCC change after including subsequent models (adding one-by-one- from 3 to 20 forming at the end 20-models-based profile) were prepared (Figure 3, Additional file 3: Figure S3).
Mixed Nafion®/silicon oxide membranes with non-uniform Si/S concentration ratio profile were prepared by allowing tetraethoxysilane (TEOS) and HF to diffuse simultaneously from isopropanol solutions located at opposite sides of the ionomer membrane.
First, in order to examine how the surface roughness of a fracture would affect its permeability characteristics, three types of specimens having different surface roughness profiles were prepared (Specimens J055, J107, and J200), which have JRC values of 5.5, 10.7, and 20.0, respectively.
The GRADE evidence profiles were prepared by one reviewer (NO) and verified independently by two reviewers (ME and DS).
The GRADE evidence profiles were prepared by one reviewer (NO) and verified independently by a second reviewer (ME).
The condensed disease profiles were prepared on the basis of the comprehensive list of symptoms (Additional file 1; File S5).
Fatty acid profiles were prepared by a slight modification of a method previously described by Yazici et al. [ 23].
Using purified and live-frozen CD4+ and CD8+ lymphocytes (in the range of 365 500,000 cells per IP), H3K4 trimethylation profiles were prepared from each individual.
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