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However, the post-transcriptional profiles of transcripts such as Ddx17, Slc1a15, or Cpeb1, could be suggestive of rhythmic miRNA activity.
Real-time PCR was used to validate expression profiles of transcripts using the Roche LightCycler 480 relative quantification software.
The human genome sequence and expression profiles of transcripts in the inner ear have aided positional cloning approaches.
Thus the arrays were designed to study global gene expression profiles of transcripts relevant to the molting process, across the entire molt cycle.
Additionally, the expression profiles of transcripts involved in phytohormone metabolism and symbiotic nodulation signal transduction were also measured by qRT-PCR (Additional files 10 and 11).
A holistic approach to examine differential gene expression profiles of transcripts relevant to the moulting process, across all moult cycle stages, was used in this study.
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In our study, the profiles of transcript abundance of TaVRN1, the proposed major promoter of flowering, were entirely consistent with those reported in other studies [ 15, 20, 21].
Principal components analysis (PCA) was used to examine the global profiles of transcript abundance of the four B. rapa genotypes across six seed developmental stages.
In order to identify common profiles of transcript abundance across the seed development stages, genes were grouped using hierarchical cluster analysis with Euclidean distance of normalized data (∆∆CT).
Consistently with the steadiness of growth, the profiles of transcript density were thus assumed to be steady for a few days in the following (see also Additional File 2: Figure S2B for additional indirect arguments).
Genes in groups VI and IX have opposite profiles of transcript accumulations between WT and jai1-1 peating at stage 3, which shows the maximum JA/JA-Ile levels in WT stamens.
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